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What about the sensitivity? Isn’t a PCR based on RNA (ie RT-qPCR) more sensitive than a PCR on DNA (qPCR)?

If metabolically active cells are present, then detecting RNA lowers the detection limit of an assay. However the analysis is then no longer quantitative as it is unknown if there are many inactive cells present, or just a few active ones. It depends on the use case whether this is necessary or not.

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